ABSTRACT: Fluorescent proteins photoswitchable with noncytotoxic light irradiation and spectrally distinct from multiple available photoconvertible green-to-red probes are in high demand. We have developed a monomeric fluorescent protein, called PSmOrange2, which is photoswitchable with blue light from an orange (ex./em. at 546 nm/561 nm) to a far-red (ex./em. at 619 nm/651 nm) form. Compared to another orange-to-far-red photoconvertable variant, PSmOrange2 has blue-shifted photoswitching action spectrum, 9-fold higher photoconversion contrast, and up to 10-fold faster photoswitching kinetics. This results in the 4-fold more PSmOrange2 molecules being photoconverted in mammalian cells. Compared to common orange fluorescent proteins, such as...
Fluorescent proteins are intrinsically fluorescent, genetically encodable tags that can be expressed...
We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-m...
The use of green fluorescent protein (GFP) mutants has represented a revolution in the application o...
Fluorescent proteins photoswitchable with noncytotoxic light irradiation and spectrally distinct fro...
We report a monomeric PSmOrange protein that is initially orange (excitation and emission at 548 and...
<div><p>A structural analysis of the recently developed orange fluorescent proteins with novel pheno...
Reversibly photoswitchable fluorescent proteins (RSFPs) are fluorescent proteins whose fluorescence,...
International audiencePhototransformable fluorescent proteins (FPs) have received considerable atten...
SummaryWe have developed the first red fluorescent protein, named rsTagRFP, which possesses reversib...
A structural analysis of the recently developed orange fluorescent proteins with novel phenotypes, L...
We have developed the first red fluorescent protein, named rsTagRFP, which possesses reversibly phot...
We report that photoconversion is fairly common among orange and red fluorescent proteins, as a scre...
International audienceFluorescent proteins have revolutionized life sciences because they allow noni...
The performance of Förster Resonance Energy Transfer (FRET) biosensors depends on brightness and pho...
A structural analysis of the recently developed orange fluorescent proteins with novel phenotypes, L...
Fluorescent proteins are intrinsically fluorescent, genetically encodable tags that can be expressed...
We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-m...
The use of green fluorescent protein (GFP) mutants has represented a revolution in the application o...
Fluorescent proteins photoswitchable with noncytotoxic light irradiation and spectrally distinct fro...
We report a monomeric PSmOrange protein that is initially orange (excitation and emission at 548 and...
<div><p>A structural analysis of the recently developed orange fluorescent proteins with novel pheno...
Reversibly photoswitchable fluorescent proteins (RSFPs) are fluorescent proteins whose fluorescence,...
International audiencePhototransformable fluorescent proteins (FPs) have received considerable atten...
SummaryWe have developed the first red fluorescent protein, named rsTagRFP, which possesses reversib...
A structural analysis of the recently developed orange fluorescent proteins with novel phenotypes, L...
We have developed the first red fluorescent protein, named rsTagRFP, which possesses reversibly phot...
We report that photoconversion is fairly common among orange and red fluorescent proteins, as a scre...
International audienceFluorescent proteins have revolutionized life sciences because they allow noni...
The performance of Förster Resonance Energy Transfer (FRET) biosensors depends on brightness and pho...
A structural analysis of the recently developed orange fluorescent proteins with novel phenotypes, L...
Fluorescent proteins are intrinsically fluorescent, genetically encodable tags that can be expressed...
We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-m...
The use of green fluorescent protein (GFP) mutants has represented a revolution in the application o...