Theor Appl Genet (2003) 107:1362–1374 DOI 10.1007/s00122-003-1398-6

Abstract A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the posi-tions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to link two existing RFLP-based genetic maps with a base map developed for the Mesoamerican Andean population, DOR364 G19833. Two types of microsatellites were mapped, based respectively on gene-coding and anonymous ge-nomic-sequences. Gene-based microsatellites proved to be less polymorphic (46.3%) than anonymous genomic microsatellites (64.3%) between the parents of two inter-genepool crosses. The majority of the microsatellites produced single bands and detected single loci, however four of the gene-based and three of the genomic microsatellites produced consistent double or multiple banding patterns and detected more than one locus. Microsatellite loci were found on each of the 11 chromosomes of common bean, the number per chromo-some ranging from 5 to 17 with an average of ten microsatellites each. Total map length for the base map was 1,720 cM and the average chromosome length was 156.4 cM, with an average distance between microsatel-lite loci of 19.5 cM. The development of new microsatel-lites from sequences in the Genbank database and the implication of these results for genetic mapping, quanti-tative trait locus analysis and marker-assisted selection in common bean are described