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including cell growth, cell death, pluripotency, cell differentia-n-d ta n-nof splice site selection is not simply governed by the interaction of snRNPs and non-snRNP protein factors with pre-mRNA but that factors associated with chromatin and the transcriptional genes produce alternatively spliced transcripts (Pan et al., 2008; Wang et al., 2008) and that the frequency of AS scales with cell type and species complexity (Barbosa-Morais et al., 2012;machinery are also important (Luco et al., 2011). Moreover, splicing can ‘‘reach back’ ’ to impact chromatin composition Nilsen and Graveley, 2010). The main types of AS found in eukaryotes are ‘‘cassette’ ’ exon skipping, alternative 50 and 30Analysis of human genome architecture emphasizes a major challenge for accurate recognition and regulation of splice site