Full Paper Detection of NADH and Ethanol at Titanium Containing MCM-41

Titanium-containing MCM-41 (Ti-MCM-41) modified glassy carbon electrode (GCE) can exhibit an excellent electrocatalytic activity towards the oxidation of b-Nicotinamide adenine dinucleotide (NADH). A dramatic decrease in the overvoltage of NADH oxidation reaction is observed at 0.28 V vs. SCE. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this material. The proposed sensor shows a highly sensitivity, an acceptable reproducibility and a good stability. The linear range of ethanol is 25 – 1000 mM and the detection limit is 8.0 mM. Ti-MCM-41 modified electrode not only can be used to detect the concentration of NADH in biochemical reaction, but also as the potential matrix for the construction of dehydrogenases sensor