T47D cells arrested at G2M and Hyperploidy Formation Induced by a Curcumin’s Analogue PGV-1

Pentagamavunon-1 (PGV-1) is a curcumin analogue, which is more stable in organic solution and more rigid in its chemical structure than curcumin. As a curcumin analogue, PGV-1 was considered to have anticancer activities. This research was conducted to study the effect of PGV-1 on the cycle progression of T47D cells. Cyto-toxic effects of PGV-1 on T47D cells were determined using MTT assay, and the the effect on cell cycle progression was carried out using flowcytometry. Western blot analysis was used to analyze protein expression correspond-ing to cell cycle progression. The result showed that at the concentration of 2.5 µM PGV-1 inhibited cell cycle progression through G2/M arrest and induced of cells hyperploidy formation. The hyperploidy formation in-duced by PGV-1 was related to the increase of cdc-2 expression. PGV-1 2.5 µM elevated the level of p21 CIP/KIP through p53- independent manner. Apoptosis was also induced by PGV-1 at early phase of treatment indicated by PARP cleavage due to activation of caspase-3/7 after 12 h treatment. The results above suggest that PGV-1 inhibits the growth of T47D cells targeted on microtubules