Giselbert Hauptmann (ed.), In Situ Hybridization Methods, Neuromethods, vol. 99, DOI 10.1007/978-1-4939-2303-8_2, © Springer Science+Business Media New York 2015 Chapter 2 In Situ Hybridization Methods for RNA

Visualization In C. Elegans
Gina Broitman-maduro
Morris F. Maduro

Publication date

January 2016

Abstract

A protocol is described for the detection of endogenous mRNA in whole-mount tissue using antisense RNA probes of at least 400 bases in length. The protocol takes a few days and is inexpensive, yet sensitive enough to detect low-abundance transcripts. Although optimized for the nematode C. elegans, the proto-col should work on similar tissue. Key words Whole-mount in situ hybridization, Transcript detection, Fixation, Antisense RNA probe

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Giselbert Hauptmann (ed.), In Situ Hybridization Methods, Neuromethods, vol. 99, DOI 10.1007/978-1-4939-2303-8_2, © Springer Science+Business Media New York 2015 Chapter 2 In Situ Hybridization Methods for RNA

Abstract

Extracted data

Giselbert Hauptmann (ed.), In Situ Hybridization Methods, Neuromethods, vol. 99, DOI 10.1007/978-1-4939-2303-8_2, © Springer Science+Business Media New York 2015 Chapter 2 In Situ Hybridization Methods for RNA

Abstract

Extracted data

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