Brief Communications The Transcription Factors Islet and Lim3 Combinatorially Regulate Ion Channel Gene Expression

Expression of appropriate ion channels is essential to allow developing neurons to form functional networks. Our previous studies have identifiedLIM-homeodomain (HD) transcription factors (TFs), expressedbydevelopingneurons, that are specifically able to regulate ion channel gene expression. In this study, we use the technique of DNA adenine methyltransferase identification (DamID) to identify putative gene targets of four suchTFs that are differentially expressed inDrosophilamotoneurons. Analysis of targets for Islet (Isl), Lim3, Hb9, and Even-skipped (Eve) identifies both ion channel genes and genes predicted to regulate aspects of dendritic and axonalmorphol-ogy. Significantly, some ion channel genes are bound by more than one TF, consistent with the possibility of combinatorial regulation. One such gene is Shaker (Sh), which encodes a voltage-dependent fast K channel (Kv1.1). DamID reveals that Sh is bound by both Isl and Lim3. We used body wall muscle as a test tissue because in conditions of low Ca2, the fast K current is carried solely by Sh channels (unlike neurons in which a second fast K current, Shal, also contributes). Ectopic expression of isl, but not Lim3, is sufficient to reduce both Sh transcript and Sh current level. By contrast, coexpression of both TFs is additive, resulting in a significantly greater reduction in both Sh transcript and current compared with isl expression alone. These observations provide evidence for combinatorial activity of Isl and Lim3 in regulating ion channel gene expression