Single-particle processing

This tutorial provides an introduction to the use of RELION (release-1.3) for cryo-EM structure determination. RELION is a software package that performs an empirical Bayesian approach to (cryo-EM) structure de-termination by single-particle analysis. This tutorial covers the entire single-particle analysis workflow in RELION-1.3: CTF estimation; auto-mated particle picking; particle extraction; 2D class averaging; 3D clas-sification; high-resolution 3D refinement; the processing of movies from direct-electron detectors; and final map sharpening and local-resolution estimation. Carefully going through this tutorial should take less than a day if you copy the precalculated results where indicated. After that, you should be able to run RELION on your own data. If RELION is useful in your work, please cite our papers and tell your colleagues about it