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Abbreviations used in this paper: COPII, coat protein complex II; DAF, decay-accelerating factor; Endo-H, endoglycosidase-H; ERES, ER exit sites; ERGIC, ER–Golgi intermediate compartment; EtNP, ethanolamine phosphate; GPI, glycosylphosphatidylinositol; GPI-AP, GPI-anchored protein; PE, phycoerythrin; PI-PLC, phosphatidylinositol-specific phospholipase C; VFG, VSVGex-Flag-GFP