Volume 290, number 1,2, S-60 FEBS 10167 0 1991 Federation of European Biochemical Societies 00145793/91/$3.50

The presence of a glycan of the same molecular size as the lipid linked precursor oligosaccharide (Glc,Man,GlcNAc,) of the N-linked protein glycosylation pathway in mammalian cells has been detected in a glycolipid fraction of cultured Drosophila ntefanogaster cells. Oligosaccharide sequencing studies were consistent with the existence of a glucosylated high mannose containing structure, which may be the common precursor for N-linked protein glycosylation in insect cells. N-Linked protein glycosylation; Glycolipid; Lipid-linked precursor oligosaccharide; Metabolic labelling: Drosophila n~ekogonasrer