Splice event detection and quantification from RNA-seq data

RNA-seq data can be used for transcript isoform discovery and transcript-level expression studies. Most computational tools for genome-guided transcript isoform analysis (methods that work with RNA-seq data aligned against a reference genome) fall into one of two categories: Methods for the analysis of splice events, which often rely on transcript annotation, and methods for reconstructing and quantifying full-length transcripts. SGSeq implements a method for the annotation-free detection and quantification of splice events from RNA-seq data.