Anti-typhoid Activity of Adhatoda vasica and Vitex negundo

Abstract: Typhoid activity is an acute systemic infection caused by Salmonella typhi.in present study the methanolic leafs extract of Vitex negundo and Adhatoda vasica were analyzed for anti-typhoid activity against Salmonella typhi. The leave sample was subjected to phytochemical analysis. The concentration of all the phytochemicals studied was higher in Vitex negundo than Adhatoda vasica, except alkaloids which was higher in Adhatoda vasica (11.3 ± 0.1mg/g) than Vitex negundo (8.6 ± 0.00 mg/g). Tannins were highest in both the plants, 61.3 ± 0.8 mg/g in Adhatoda vasica and 93.9 ± 0.8 mg/g in Adhatoda vasica. The antioxidant activity was determined and compared with BHA and reducing power was compared with Ascorbic acid. Both the samples had high antioxidant and reducing power activity. Several studies reported the loss of antioxidant system during infection of Salmonella typhi. The leaf extracts of Adhatoda vasica and Vitex negundo showed considerable antioxidant activity which can used as remedy against antioxidant system collapse and thus promises to be effective antioxidant supplement for typhoid patients. Besides antioxidant activity the leaves of both plants inhibited the growth of Salmonella typhi. The antibacterial activity of both leaf extracts were compared with gentamycin. The results of present shows that leaf extracts of Vitex negundo and Adhatoda vasica confer anti-typhoid activity against Salmonella typhi