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For construction and characterization of prototype devices with nanometer-scale parts, this Ph.D. project developed an in-situ scanning electron microscope (SEM) laboratory with a set of novel tools for three-dimensional nanomanipulation. Together the developed set of tools comprise a nanolaboratory which in many ways can accomplish the same tasks as an electronic workshop- but using nanoscale components that are some 10000 times smaller. The developed nano-toolbox contains microfabricated grippers that have been used successfully for pick-and-place manipulation of nanowires and grippers with integrated force-feedback. Environmental electron beam deposition (EEBD) was developed as a method for soldering electrical nanoscale circuits and constructing three-dimensional nanostructures. To characterize nanostructures in a transmission electron microscope (TEM), a method using micro-cantilever chips was developed and used to study Joule heating effects in carbon nanotubes. Tools for In-situ Manipulatio