lH NMR Model Studies of Amphotericin B: Comparison of X-Ray and NMR Stereochemical Data

‘H NMR data for amphotericin B methoxycarbonylmethylamide extracted from DQF-COSY, ROESY and 1D spectra were compared with x-ray data for amphotericin B. The conformation of amphotericin B in solution was identical with that found by x-ray analysis in the solid state. Most of the relay artifacts observed in the ROESY spectrum were recognized, and their pathways of formation were assigned. KEY WORDS Polyene macrolides Amphotericin B Solution-state conformation 2D NMR COSY ROESY INTRODUCTION RESULTS AND DISCUSSION Polyene macrolides constitute a class of antifungal agents of which over 200 reprentatives have been recog-nized, and a considerable number have been character-ized by their gross structures. ’ However, the elucidatio