Background: While intracellular buffers are widely used to study calcium signaling, no such tool exists for the other major second messenger, cyclic AMP (cAMP). Methods/Principal Findings: Here we describe a genetically encoded buffer for cAMP based on the high-affinity cAMP-binding carboxy-terminus of the regulatory subunit RIb of protein kinase A (PKA). Addition of targeting sequences permitted localization of this fragment to the extra-nuclear compartment, while tagging with mCherry allowed quantification of its expression at the single cell level. This construct (named ‘‘cAMP sponge’’) was shown to selectively bind cAMP in vitro. Its expression significantly suppressed agonist-induced cAMP signals and the downstream activation of PKA wi...
The role of cAMP as a second messenger in cellular signalling is well established. In the eukaryotic...
Termination of cyclic adenosine monophosphate (cAMP) signaling via the extracellular Ca(2+)-sensing ...
doi:10.1016/j.jmb.2007.09.035 Available online at wwwIntroduction The adenosine 3′, 5 ′ cyclic monop...
BACKGROUND: While intracellular buffers are widely used to study calcium signaling, no such tool exi...
BACKGROUND:While intracellular buffers are widely used to study calcium signaling, no such tool exis...
Cyclic adenosine monophosphate (cAMP) is a second messenger that is derived from adenosine triphosph...
AbstractToday, there is evidence that the cAMP-dependent kinases (PKA) are not the only intracellula...
Cyclic adenosine 3,'5'-monophosphate (cAMP) is the archetypal second messenger produced at the membr...
Diverse pathophysiological processes (e.g. obesity, lifespan determination, addiction and male ferti...
Diverse pathophysiological processes (e.g. obesity, lifespan determination, addiction and male ferti...
cAMP is a ubiquitous second messenger responsible for the cellular effects of multiple hormones and ...
The intracellular second messenger cyclic AMP (cAMP) is degraded by phosphodiesterases (PDE). The kn...
Intracellular fluctuations of the second messenger cyclic AMP (cAMP) are regulated with spatial and ...
Signaling networks are spatiotemporally organized in order to sense diverse inputs from the extracel...
Abstract Background cAMP is an ubiquitous second messenger mediating various neuronal functions, oft...
The role of cAMP as a second messenger in cellular signalling is well established. In the eukaryotic...
Termination of cyclic adenosine monophosphate (cAMP) signaling via the extracellular Ca(2+)-sensing ...
doi:10.1016/j.jmb.2007.09.035 Available online at wwwIntroduction The adenosine 3′, 5 ′ cyclic monop...
BACKGROUND: While intracellular buffers are widely used to study calcium signaling, no such tool exi...
BACKGROUND:While intracellular buffers are widely used to study calcium signaling, no such tool exis...
Cyclic adenosine monophosphate (cAMP) is a second messenger that is derived from adenosine triphosph...
AbstractToday, there is evidence that the cAMP-dependent kinases (PKA) are not the only intracellula...
Cyclic adenosine 3,'5'-monophosphate (cAMP) is the archetypal second messenger produced at the membr...
Diverse pathophysiological processes (e.g. obesity, lifespan determination, addiction and male ferti...
Diverse pathophysiological processes (e.g. obesity, lifespan determination, addiction and male ferti...
cAMP is a ubiquitous second messenger responsible for the cellular effects of multiple hormones and ...
The intracellular second messenger cyclic AMP (cAMP) is degraded by phosphodiesterases (PDE). The kn...
Intracellular fluctuations of the second messenger cyclic AMP (cAMP) are regulated with spatial and ...
Signaling networks are spatiotemporally organized in order to sense diverse inputs from the extracel...
Abstract Background cAMP is an ubiquitous second messenger mediating various neuronal functions, oft...
The role of cAMP as a second messenger in cellular signalling is well established. In the eukaryotic...
Termination of cyclic adenosine monophosphate (cAMP) signaling via the extracellular Ca(2+)-sensing ...
doi:10.1016/j.jmb.2007.09.035 Available online at wwwIntroduction The adenosine 3′, 5 ′ cyclic monop...