ISOZYMES AND BIO-CONTROL ANALYSIS OF FUSRIAUM SPP. FROM POST-HARVEST DISEASED MANGO

Abstract- Electrophoretic studies of Isozymes and whole cell protein using Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) was carried out to evaluate genetic diversity and intra specific variations among Fusarium solani and F.oxysporum causative fungal pathogens of mango rotting in Pakistan. In present study 47 isolates were studied for morphological variability and in vitro bio-efficiency of plants against Fusarium spp. Among them 37 isolates randomly selected for molecular analysis. Results revealed morphological characteristics of isolates were similar to each other. 24 phenotypes obtained by using four isozymes i.e. Lactate dehydrogenase (LDH), Malate dehydrogenase (MDH), Glucose-6-phosphate dehydrogenase (G6PDH), ǃ-glucosidase (ǃ-glu). Among studied isolates MDH exhibit lowest activity. Isozymes banding pattern obtained by SDS-PAGE was used to compute Jaccards similarity coefficients which ranged 0.25 to 1.0 indicating genetic diversity of the isolates. Cluster analysis with UPGMA showed isolate