In Vitro Susceptibility of Coliform and Pseudomonas Species to Some Antibiotics by E-test Strips

Antimicrobial susceptibility The aim of this study was to characterize the in vitro antimicrobial susceptibility profile of Coliform and Pseudomonas species strains to 10 antibiotics (Vancomycine, Ciprofloxine, Tetracyclin, Chloramphenicol, Erythromycin, Streptomycine, Gentamycine, Ampicilline, trimethoprim-sulfamethoxazole and Clarithromycin) that could potentially be used in clinical therapy. MIC was determined for antibiotics in Mueller-Hinton (MH) agar plates, by using E-test strips (Liofilchem, Roseto degli Abruzzi, Italy). MIC was read after 24 h of incubation at 37°C. The both strains were more susceptible to Ciprofloxine (MIC values: Coliform, 0.002 µg/mL; and Pseudomonas, 0.047 µg/mL). No strain of Pseudomonas was found sensitive to Chloramphenicol, Erythromycin, Ampicilline, trimethoprim-sulfamethoxazole and Clarithromycin, contrary to coliform, which was found resistant to those antibiotics with MIC values ranged between 0.38 and 128 µg/mL