CLINICAL EVALUATION OF THE EFFICACY OF PYRIDOXAL-5'-PHOSPHATE AND MAGNESIUM IN PREVENTION OF GLYCINE INDUCED UROLITHIASIS IN MALE RATS

Abstract- The objective of the present study was to assess the efficacy of high level of pyridoxal-5'-phosphate (PLP) and magnesium in the form of magnesium oxide (MgO) at low and high levels in the prevention of urolithiasis induced by high dose of glycine in male albino rats. Seven groups each of ten adult male Albino rats (Sprague-Dawely) strain, mean weight varied between 97.8 to 100.2 g were fed on either basal control diet or experimental diets containing high level of glycine (3%) to induce urolithiasis and supplemented with PLP at high dose (0.7%) alone or incorporated with MgO at two levels (0.25 % and 0.5%) throughout two successive experimental periods (15 and 30 days). 24h urine samples and serum were analyzed for biochemical indicators of kidney function. The results showed that the supplementation of both PLP and MgO to the urolithic rats decreased the urinary excretion of oxalate, calcium, phosphates, uric acid, urea, and total proteins and increased the excretion of magnesium and citrates. Serum levels of creatinine, albumin, total cholesterol, potassium, sodium, PTH, phosphates, and ALP activity were decreased and the activities of LDH and G6PD were increased in treated rats by PLP and MgO at high doses throughout two successive experimental periods. The present study concluded that, urolithiasis and renal calcium oxalate deposition induced by high dose of glycine (3%) in male albino rats could be completely prevented by diets high in magnesium (0.5%) and partially prevented by very high dietary levels of PLP (0.7%). The combined supplementation of PLP and Mg to experimental rats improved urinary functions and increased the excretion of urinary citrate and magnesium more than either supplement alone