©ARC Page | 75 In Silico Drug Search for Better Treatment for Cancer: L-Asparaginase

Abstract: L-Asparaginase is required by normal cells for the utilization of the amino acid arginine. When the normal cell turns to cancerous, it cannot synthesis arginine, but it can extract the amino acid from external supply in the plasma and tissue. In such instance if we supply the L-asparaginase as drug it will destroy the supplied arginine and the cancer cell will die due to starvation. The source of L-asparaginase is different. It may be fungi, bacteria, plants and animals. Here we made an attempt to study the possible better source of L-asparaginase for the treatment of cancer by docking method. The docking study reveals that L-asparaginase is better as drug from 12 different sources we have studied. Erwinia carotovora’s L-asparaginase shows-62. 83 energy this is least from all the selected 12 sources of organisms. This followed by Campylobacter jejuni an all other sources shown more or less same energy level higher that these two sources. The other parameters studied like Lipinski data and other drug parameters show that asparaginase from Erwinia carotovora is better than other sources in the sense of drug candidates. We also studied physico-chemical parameters, trans-membrane sequence prediction, secondary structure prediction of the sequence extracted from the source, 3D model preparation and its validation. The conclusion drawn from the study should be validated by further study and validation in animal models and human