Research Article IN VIVO AND IN VITRO BIOCHEMICAL ESTIMATION OF PRIMARY METABOLITES FROM

Medicinal plants are the most exclusive source of life saving drugs for the majority of the world’s population. Since time immemorial, man has used various plant parts in the treatment and prevention of various ailments. They are of great interest to the researchers in the field of biotechnology as most of the drug industries depend, in part, on plants for the production of pharmaceutical compounds. Plants provide biologically active metabolites and lead structures whose activities can be enhanced by manipulation through combinations with chemicals and synthetic chemistry. These metabolites can be exploited in the field of new drugs research and for the development of modified derivatives with enhanced activity and reduced toxicity. In present investigation, callus raised from leaf explant and different plant parts (leaves, stems and roots) of Jatropha curcas Linn. were evaluated for their biochemical estimation of primary metabolites quantitatively. Results showed that maximum callus was obtained on MS medium supplemented with a combination of IAA+ Kn. Maximum content of soluble sugars and proteins were found in callus and starch, lipid and phenolic contents were found in leaf. It further concludes that Jatropha curcas serves as a rich source of primary metabolites which can be used as raw material in industries