Article history Received

The present study aims, to develop analytical method of isolated constituent in methanolic extract of leaves of Millingtonia hortensis linn. Development of new methods for routine analysis of various chemical constituents present in plants is highly demanded by the industry. The extracts of Millingtonia hortensis linn were obtained by continuous heat extraction method by using Soxhlet extraction process containing methanol as solvents. Extract was further used for phytochemical study. Phytochemical screening showed presence of flavonoids and triterpenoid. TLC, IR, GCMS analysis of methanolic extracts shown new chemical constituents Rutin. The developed HPLC method includes use of C18 Intersil, 4.6 (i.d.) x 250 mm column, mobile phase consist of mixture of methanol: Water (pH 5.5) in the ratio of 85: 15 % v/v at flow rate.5 ml / min. Retention time was found to be 4.55, 4.52 for isolated Rutin and standard rutin respectively. Therotical plates was found to be 8045, 8234 for isolated and standard rutin. Method was validated with the help of parameter as linearity, range, accuracy, precision (intraday and interday’s), LOD, LOQ. The developed method was found to be a relatively simple, precise and reproducible for the quantification of Rutin. The method does not employ any derivatization procedure and can be used as a quality control tool for the routine analysis of rutin from leaves extract. Correspondence to Author