www.ijcrcps.com Research Article THE EFFECT OF GALLIC ACID, NARINGIN, CHRISIN AND QUERCETIN AS FLAVONOIDS, ON THE THERMODYNAMIC STABILITY OF TYROSINASE

Background: Tyrosinase is a copper-containing monooxygenase responsible for the biosynthesis of melanins and other polyphenolic compounds. Since tyrosinase function lead to the different biological process through animals and its deficiency may cause problems, thus the study of its activation or stabilizations is as important as its inhibition. Method: The thermodynamical stability and inhibition of mushroom tyrosinase (MT) from Agaricus Bisporus were investigated in the presence of gallic acid, naringin, chrisin, quercetin. Therefore, the protein denaturation and thermal scanning was studied. Sigmoid denaturation curves were analysed according to the two models of Pace theory to obtain the Gibbs free energy change of denaturation process. Results and conclusion: In the presence of gallic acid, kinetic assessment of the enzyme activity showed a non-competitiv