www.ijcrcps.com Research Article COMPARATIVE ANALYSIS OF LECTINS ISOLATED FROM SEED AND TESTA OF

Artocarpus heterophyllus or jackfruit is the national fruit of Bangladesh. The objective of this study is to characterize and compare the lectin proteins isolated from seeds and testa of Artocarpus heterophyllus. Thin layer chromatography was used to detect the presence of protein in seeds and testa.Hemagglutination assay was done to identify the isolated proteins as lectins. The lectin profiles of seeds and testa were analyzed using SDS-PAGE technique. Antioxidant effects were measured by DPPH scavenging assay. Anti-inflammatory assay was also investigated.Carbohydrate induced and bacterial induced hemagglutination inhibition was done. The seed lectin showed stronger hemagglutination activity and antioxidant activity compared to testa lectin. However, testa lectin showed potent anti-inflammatory activity at low concentration compared to seed lectin. Therefore, in the future these lectins may have the potential to play role as biotechnological tools