www.ijips.net INTERNATIONAL JOURNAL OF INVENTIONS IN PHARMACEUTICAL SCIENCES DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR THE ESTIMATION DIVALPROEX SODIUM IN TABLETS

ABSTRACT: A simple, sensitive, precise and robust reverse-phase high-performance liquid chromatographic method for analysis of divalproex sodium in pharmaceutical formulation such as tablets was developed and validated as per ICH guidelines. The chromatographic system employs isocratic elution using on Agilent SB C18, (150 x 4.6 mm, 5m) with Buffer and Acetonitrile were mixed in the ratio 60:40 v/v, added drop wise, as mobile phase set at flow rate 1.0 ml / min. The standard curve was found to have a linear relationship (r2> 0.99) over the analytical range of 10–150µg/ml. The Percentage Assay values were calculated for both the drugs and found to be in between 98.0 %- 102.0%. The % Recovery values for Valproic acid were found to be in between 98.0 % to 102.0%. Therefore the rapid and sensitive developed method can be used for the development and estimation of divalproex sodium in tablets