www.ijcrcps.com Research Article EFFECT OF SILVER NANOPARTICLES ON THE GROWTH OF BACTERIAL ISOLATES FROM URINARY CATHETERS AND INTRAUTERINE DEVICES

The mechanism of prevention of bacterial growth by antibiotics is quite different from the mechanisms by which nanoparticles inhibit microbial growth. But in the present study effect of silver nanoparticles on the isolates from catheter and intra uterine devices biofilms was studied. Also invitro studies were carried out to find microbial biofilm formation on catheter tips and IUDs. Silver nanoparticles in aqueous solution were prepared by reduction of AgNO3 with NaBH4 and stabilized by using tri sodium citrate. The nanoparticles obtained hereby are stable for weeks. Microorganisms were isolated and were sub cultured in Muller Hinton broth for 24h at 37oC. Wells of 6mm diameter were made on Muller Hinton agar plates using gel puncher. This study shows that the biofilm isolate P. mirabilis is very sensitivity to silver nanoparticles than standard antibiotics. The sensitivity of Neisseria gonnorrhaea to silver nanoparticles is little less. This can be seen from the diameter of zone of inhibition. So infections in patient with individually catheter and IUDs are difficult to treat with high dose of antibiotics. As silver nanoparticles even at a small dose inhibit the bacterial colonisation these particles can be coated on the devices to inhibitory bactericidal concentration