Research Article HPTLC Analysis of Flavonoid Compound Profile in the whole-plant Methanol Extract

A comparison of HPTLC analysis was carried out to record the flavonoid compounds profile in the whole-plant samples of three Polygonum species (P. chinense, P. glabrum and P. barbatum). The methanol extract of whole-plant samples obtained from P. chinense, P. glabrum and P. barbatum shows 8, 6 and 10 compounds, respectively, and were compared with rutin and apigenin standards. Among the compounds, 2 compounds in each sample were identified as flavonoids while the others were unknown. One flavonoid compound from each of P. chinense and of P. barbatum showed same peak Rf values (0.38). Similarly, one unknown compound from P. glabrum and P. barbatum were also showed same peak Rf values (0.97) while all other compounds are differ from each other. All the three Polygonum species tested differ in their nature and number of flavonoid compounds detected