Polymorphisms in Turkish Patients with Nonsyndromic Cleft Lip and Palate Research Article

Objective: To assess the association between the MTHFR gene (Al298C), MTR gene (A2756G) and MTRR gene (A66G) polymorphisms and nonsyndromic cleft lip with or without cleft palate(nsCL/P), we investigated patients in the Turkish population. Methodology: Analysis of gene polymorphisms was carried out using polymerase chain reactions and restriction enzyme digestions. Our study included 100 patients with nsCL/P and 125 age matched healty individuals. Results: We found that MTHFR Al298C polymorphism and MTR A2756G polymorphism are significant risk factors for nsCL/P. The frequency of MTRR A66G homozygotes was higher compared to control group, however the difference was not statistically significant. Conclusions: We revealed a statistical significant association between the MTHFR Al298C and MTR A2756G polymorphism and nsCL/P. Nonsyndromic cleft lip with or without cleft palate (nsCL/P, MIM 119530) is one of the most common birth defects, with a birt