Modified gel preparation for distinct DNA fragment

Abstract. Agarose gel electrophoresis is the standard method that is used to separate, identify, and purify DNA fragments. However, this method is time-consuming and capable of separating limited range of fragments. A new technique of gel preparation was developed to improve the DNA fragment analysis via electrophoresis. Typically, agarose gels are used to separate larger fragments of DNA (greater than 200 bp) and polyacrylamide gels are used for small fragments (less than 200 bp). The distance between two DNA fragments of different sizes can be determined based on the concentration of the agarose matrix (Sambrook et al., 1989). The relative mobility of DNA depends primarily on the agarose concentration in the gel, strength of the applied current, the ionic strength o