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Technologies for the production of biofuels have increased attention due to the rising cost of petrol and global warming. One such technology under development is the use of Lactobacillus pentosus for biofuels production. Lactobacillus pentosus is lactic acid bacteria which able to metabolize both of pentose and hexose, and to grow on media containing hydrolyzed biomass. Thus it has many advantages as host for multiple purposes such as micro bioenergy development. To develop the strain, we already cloned gene pdc and adh2 from zymobacter palmae and Lactobacillus plantarum, respectively. In this paper, we will report our strategy to develop those microbioenergy and our recen research progress