Enhancing Meniscus Repair through Biomaterial Design

The knee meniscus is prone to damage, which leads to pain and inhibits mobility in the joint long term. Due to the minimal vascularity, low cellularity and large mechanical forces imparted on the meniscus with normal use, endogenous repair is limited. Resection of the damaged region of the tissue (meniscectomy) remains the most common treatment for a torn meniscus, but this procedure results in cartilage degradation and other adverse changes in the knee joint. Given the prevalence of meniscus damage, there is thus a pressing need for novel approaches to meniscus repair. To address this issue, this thesis developed in vitro techniques to analyze the time-varying properties of the aging meniscus, and to address how the meniscus repair interface might be modulated through the use of growth factors. Further, electrospun scaffolds were designed to replicate key architectures of the native tissue while providing controlled release of biologic factors. Our findings demonstrated marked biological, biochemical, and structural changes in meniscus with age. These findings pointed to key factors that could play a role in meniscus integration (ie repair) capacity after meniscus injury; these factors were evaluated in the context of meniscus repair using a mechanical in vitro model. To address situations where substantial meniscus tissue would be removed, we tested the integration capacity of electrospun scaffolds with native tissue and maturation of these scaffolds in response to growth facto