MICROPROPAGATION, REGENERATION AND EVALUATION OF ANTIMICROBIAL ACTIVITY OF LIPPIA NODIFLORA

Lippianodiflora (L) is an important medicinal plant used in the treatment of diseases such as Chronic indolent ulcer, gastrointestinal and respiratory disorders, diabetes etc. In the present study Nodes, internodes and leaves were used as explants for the initiation of root, shoot and callus and cultured on Murashige and Skoog (Ms) medium supplemented with different growth regulators like Indole-3-acetic acid(IAA), 2,4-dichlorophenoxy acetic acid ( 2,4-D), α-Naphthalene acetic acid(NAA) and Benzyl adenine(BAP). The callus and root were induced in medium supplemented with IAA (1mg/m) l and shoot were induced in BAP medium (1mg/ml). Antimicrobial activity were studied in different solvent extracts (acetone, chloroform, ethyl acetate, hexane and methanol) by well diffusion method against human pathogenic microorganisms Bacillus subtilis, Salmonella typhi, Staphylococcus aureus, Escherichia coli, Vibrio cholera and Candida krusei. The study also concluded that the antimicrobial activity against all the microorganism increase with increased concentration of the extract. Highest antimicrobial activity were observed in hexane stem extract (27.77%) against S. aureus, chloroform leaf extract (26.66%) against E. coli, ethyl acetate leaf extract ( 22.22%) against E.coli and S. typhi, acetone leaf and stem extracts were effective (23.33%)against B. subtilis and S. aureus respectively. The ethyl acetate extract of callus were effective against C. krusei and B. subtilis than the wild extract.Henc