Analysis and Engineering of Metabolic Pathway Fluxes in Corynebacterium glutamicum

Dedicated to Prof. Dr. Dietmar Hempel on the occasion of his 65th birthday Abstract The Gram-positive soil bacterium Corynebacterium glutamicum was discovered as a natural overproducer of glutamate about 50 years ago. Linked to the steadily increasing economical importance of this microorganism for produc-tion of glutamate and other amino acids, the quest for efficient production strains has been an intense area of research during the past few decades. Efficient produc-tion strains were created by applying classical mutagenesis and selection and especially metabolic engineering strategies with the advent of recombinant DNA technology. Hereby experimental and computational approaches have provided fascinating insights into the metabolism of this microorganism and directed strain engineering. Today, C. glutamicum is applied to the industrial production of more than 2 million tons of amino acids per year. The huge achievements in recent years, including the sequencing of the complete genome and efficient post genomic approaches, now provide the basis for a new, fascinating era of research – analysis of metabolic and regulatory properties of C. glutamicum on a global scale toward