The Fabrication of Screen Printed Electrode Mixed

In this paper, the screen printed electrode mixed ferrocenemethanol(Fc-OH) and thionin was prepared. Fc-OH acted as the oxidant and thionin as the electron transfer, the electrochemical oxidized behavior of β-hydroxybutyrate at the screen printed electrode was investigated in the present of β-hydroxybutyrate dehydrogenase and nicotinamide adenine dinucleotide (NAD). Moreover, we built a new method to detect β-hydroxybutyrate. The linear relationship of oxidized current at potential of 0.3 V with the concentration of β-hydroxybutyrate in the range of 50 μM to 5 mM was obtained by chronoamperometry (I-t), and the detection limit was 16 μM. The electrode was successfully applied to detect β-hydroxybutyrate in real samples without interference from AA and ACP.The preparation of screen printed electrode was simple, good reproducibility