, Seong Beom Jin 3

The purpose of this study was to evaluate the methods of identifying zygotic seedlings of crosses be-tween ‘Morita unshiu ’ (Citrus. unshiu Marc.) and ‘Ponkan ’ (C. reticulata Blanco). In order to investigate the frequency and position of zygotic seedlings and to determine the efficiency of zygotic seedling identification, random amplified polymorphic DNA (RAPD) and sequence-related amplified poly-morphism (SRAP) were performed using UBC (9, 27, 229, 230, and 254) primers and F4/R27, F7/R14, F12/R10, and F44/R62 primer sets, respectively. A total of 37 putative zygotic seedlings out of 55 in-dividuals were selected by RAPD and SRAP. The F7/R14 primer pair showed a screening ability of 45.5 % (25/55), whereas the primer UBC27 revealed the highest efficiency of zygotic seedling identi-fication (50.9%, 28/55). When both UBC27 and F7/R14 were properly used for selection of hybridized seedlings of ‘Morita unshiu ’ (C. unshiu Marc.) and ‘Ponkan ’ (C. reticulata Blanco), screening efficiency was increased to 60 % (33/55) for putative zygotic seedlings. Thus, it is possible to select putative hy-brid zygotic seedlings in an accurate and effective manner by RAPD and SRAP