ORIGINAL ARTICLE Dysregulated expression of lipid storage and membrane dynamics factors in Tia1 knockout mouse nervous tissue

Abstract During cell stress, the transcription and translation of immediate early genes are prioritized, while most other mes-senger RNAs (mRNAs) are stored away in stress granules or degraded in processing bodies (P-bodies). TIA-1 is an mRNA-binding protein that needs to translocate from the nucleus to seed the formation of stress granules in the cytoplasm. Because other stress granule components such as TDP-43, FUS, ATXN2, SMN, MAPT, HNRNPA2B1, and HNRNPA1 are crucial for the motor neuron diseases amyotrophic lateral sclerosis (ALS)/ spinal muscular atrophy (SMA) and for the frontotemporal de-mentia (FTD), here we studied mouse nervous tissue to identify mRNAs with selective dependence on Tia1 deletion. Transcriptome profiling with oligonucleotide microarrays in comparison of spinal cord and cerebellum, together with inde-pendent validation in quantitative reverse transcriptase PCR an