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In the northwestern region of Russia, winter cereals very often can’t realize advantages with respect to yield owing to a complex set of unfavorable overwintering conditions. At the same time, spring triticale can be grown here as an exceptionally promising feed grain crop. The selection of high-yielding forms well adapted to local conditions from heterogeneous accessions of spring triticale of the VIR (Vavilov All-Russian Research Institute of Plant Industry) world collection is effective for accelerated creation of the required culti-vars. In this case, it is necessary to analyze the structure of the initial population and it dynamics during selec-tion for a set of valuable traits. Along with field assess-ments, such a check is possible by means of molecular markers, including protein ones (electrophoretic spec-tra of gliadin) [1–3]. As a result of research at VIR’s Pushkino branch (Leningrad oblast, 1999–2001) of more than 200 spring triticale accessions of various ecogeographic prove-nance, a Mexican accession Merino/Jlo/Zebra32 (k-3503) was revealed which combines an acceptable length of the growth period (90–95 days) with high productivity, excellently filled grain, and other valuable traits. With respect to disease and lodging resistance, grain number per spike, and grain size and weight per unit area, it far surpassed the spring soft wheat cultivar Leningradka. A study of the progenies of individual plants of this accession showed the possibility of selecting forms with improved characteristics with respect to a number of traits. In particular, heterogeneity for brown rust resistance, length of the sprouting–heading stage, pho-toperiod sensitivity, and certain parameters of the spike was determined. The seeds of the best lines obtained as a result of threefold individual selection were united and gave rise to a new cultivar, Zolotoi Grebeshok. These lines were propagated and tested also individu