Study on Electrochemical Behavior of Horseridash peroxidase Enzyme by MgO Nanoparticles Modified Electrode to Identify Hydrogen peroxide

In this research, the electrochemical behavior of Horseridash peroxidase (HRP) enzyme was studied by use of modified carbon paste electrode with Magnesium oxide (MgO) nanoparticles to identify Hydrogen peroxide. Triple electrode system was used in applied electrochemical cell as carbon paste electrode was exploited in forms of bare or modified with MgO nanoparticles as working electrode; saturated calomel electrode (SCE) was used as reference electrode, and platinum electrode was applied as counter electrode. Morphological studies and analyzing the levels of synthesized MgO nanoparticles levels with visible/ultraviolet double beam spectrophotometer, X-ray diffraction and electron microscope. Cyclic voltamograms (CVs) in 10-240 mV distance within phosphate buffer solution 0.1 M (pH=7.0) were studied. Formal potential (E 0) was considered 119 ± 1 mV in contrast with reference electrode (SCE). In designed biosensor, the linear correlation of cathodic peak on different hydrogen peroxide concentrations was measured in 50-400 µm distance