Enzyme working solution:

solution to Enzyme A to prepare Enzyme working solution. * Enzyme A and B vials are capped under vacuum pressure. Add deionized water or solution through a rubber septum with a syringe, and then remove the septum. * The Enzyme working solution is stable at-20oC for 2 weeks. If store in a refrigerator, stable for 3 days. Indicator working solution: Dissolve Enzyme C and Coenzyme with 3 ml deionized water each. Add 2.8 ml Enzyme C solution and 2.8 ml Coenzyme solution to Indicator solution to prepare Indicator working solution. * Enzyme C and Coenzyme vials are capped under vacuum pressure. Add deionized water through a rubber septum with a syringe, and then remove the septum. * The Indicator working solution is stable at-20 oC for 2 weeks. If store in a refrigerator, stable for 3 days