ORIGINAL ARTICLE Autologous Cell-Enriched Fat Grafting for Breast Augmentation

Abstract Autologous fat grafting for breast augmentation has faced some historical hurdles. However, in recent years it has been gaining acceptance from the medical commu-nity. This prospective, nonrandomized open-label study of 20 Japanese women supports the use of autologous fat grafting in breast augmentation and explores enhancement of fat graft tissue with autologous adipose-derived regen-erative cells (ADRCs). After adipose harvesting using syringe liposuction, the tissue is processed in the Celution 800 System, which washes the graft and isolates ADRCs. The average cells per gram of harvested adipose tissue was 3.42 9 105, and the mean cell viability measured using an automated cell counting system before graft delivery was 85.3%. All patients demonstrated improvement in cir-cumferential breast measurement (BRM) from their base-line state, and breast measurements were stable by 3 months after surgery. The mean BRM 9 months after surgery had increased 3.3 cm from preoperative measure-ments. Through 9 months, overall physician satisfaction was 69 % and patient satisfaction was 75%. No serious or unexpected adverse events were reported, and the proce-dure was safe and well tolerated in all patients. Postoper-ative cyst formation was seen in two patients. These prospective results demonstrate that ADRC-enriched fat grafts processed with a closed automated system maintain high cell viability and that the procedure is safe and effective, with all patients showing improvement after a single treatment