Automated, high-throughput platform for protein solubility screening using a split-GFP system

Overproduction of soluble and stable proteins for functional and structural studies is a major bottleneck for structural genomics programs and traditional biochemistry laboratories. Many high-payoff proteins that are important in various biological processes are “difficult to handle ” as protein reagents in their native form. We have recently made several advances in enabling biochemical technologies for improving protein stabilit