1 Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR

Computer programs: (Programs with bold names are initiated by the user, other programs are initiated by the programs with bold names) MVdPCR_DataInput.m- This program is used to analyze experimental data, which has been entered into Datainput.xls. It reads Datainput.xls to obtain parameters and experimental results, then calculates and using MVdPCR_MLE.m. For experimental results being tested for statistical significance, the Z-score (Eq. 14) and confidence level are also determined. 2 MVdPCR_MLE.m- This program uses a globalized Newton method to calculate the MPN, by solving for in Eq. 8, for the parameters input from MVdPCR_DataInput.m or MVdPCR_Simulate.m. It also calculates using Eq. 12. MVdPCR_Simulate.m- This program uses the input parameters from MVdPCR_RunSim, MVdPCR_Plot_points, or MVdPCR_Find_device_lower.m to generate simulated results. These results are then analyzed through MVdPCR_MLE.m, and the power level for the set of results are determined. MVdPCR_RunSim.m- This program provides the input parameters for MVdPCR_Simulate.m for specific input concentrations and resolution levels