Dynamics of Protein Folding and Cofactor Binding Monitored by Single-Molecule Force Spectroscopy

to ime in ging n e G6 the ldin jor wa enc ns roles of cofactors in the functions of these proteins, but may also offer new insights into understanding of the binding mechanisms. Therefore, using single-molecule methodologies to directly probe different conformers during monitor the kinetics of protein folding and its cofactor AFM can readily monitor the evolution of different protein conformers during the folding of proteins in the presenc