Sensitivity analysis and robust experimental design of a signal transduction pathway system

ABSTRACT: Experimental design for cellular networks based on sensitivity analysis is studied in this work. Both optimal and robust experimental design strategies are developed for the IκB-NF-κB signal transduction model. Based on local sensitivity analysis, the initial IKK intensity is calculated using an optimal experimental design process, and several scalarizationmeasures of the Fisher informationmatrix are compared. Global sensitivity analysis and robust experimental design techniques are then developed to consider parametric uncertainties in the model. The modified Morris method is employed in global sensitivity analysis, and a semidefinite programmingmethod is exploited to implement the robust experimental design for theproblem of measurement set selection. The parametric impacts on the oscillatory behavior of NF-κB i