A Time-Resolved Fluorescence Immunoassay for the Measurement of Testosterone in Saliva: Monitoring of Testosterone Replacement Ther- apy with

Monitoring of testosterone replacement therapy re-quires a reliable method for testosterone measure-ment. Determination of salivary testosterone, which reflects the hormone’s biologically active plasma frac-tion, is a superior technique for this purpose. The aim of the present study was to establish a new sensitive time-resolved fluorescence immunoassay for the accu-rate measurement of testosterone levels in saliva and to validate it by monitoring testosterone replacement therapy in eight hypogonadal men. A clinical phase I- study with the new ester testoste-rone buciclate was performed to search for new tes-tosterone preparations to produce constant serum levels in the therapy of male hypogonadism. After two control examinations eight male patients with primar