Mapping of the mouse hyh gene to a YAC/BAC contig on proximal

Abstract. Mice that are homozygous for the autosomal recessive hydrocephaly with hop gait (hyh) mutation on Chromosome (Chr) 7 have congenital hydrocephalus characterized by an interhemi-spheric cyst arising from the third ventricle and agenesis of the corpus callosum. Analysis of more than 500 backcross and inter-cross progeny maps the hyh locus to proximal Chr 7, approxi-mately 13 cM centromeric to its originally reported map position. Analysis of recombinants at several MIT microsatellite markers localized the hyh locus between D7Mit75 and D7Mit56. Develop-ment of several new SSLP markers allowed us to refine the hyh candidate interval to a region defined by the cone-rod homeobox (Crx) gene proximally and D7Mit56 distally. A contig of yeast artificial chromosome (YAC) clones and bacterial artificial chro-mosome (BAC) clones spanning this entire region has been devel-oped, and a number of potential candidate genes for hyh within thi