Capillary electrophoresis Chondroitin/dermatan sulfate

a b s t r a c t A quantitative and highly sensitive method for the analysis of glycosaminoglycan (GAG)-derived disac-charides that relies on capillary electrophoresis (CE) with laser-induced fluorescence detection is pre-sented. This method enables complete separation of 17 GAG-derived disaccharides in a single run. Unsaturated disaccharides were derivatized with 2-aminoacridone to improve sensitivity. The limit of detection was at the attomole level and approximately 100-fold more sensitive than traditional CE–ultra-violet detection. A CE separation timetable was developed to achieve complete resolution and shorten analysis time. The relative standard deviations of migration time and peak areas at both low and high concentrations of unsaturated disaccharides are all less than 2.7 and 3.2%, respectively, demonstrating that this is a reproducible method. This analysis was successfully applied to cultured Chinese hamster ovary cell samples for determination of GAG disaccharides. The current method simplifies GAG extraction steps and reduces inaccuracy in calculating ratios of heparin/heparan sulfate to chondroitin sulfate/der-matan sulfate resulting from the separate analyses of a single sample