Add to ORKGBuffalo oocytes In vitro maturation In vitro fertilization Somatic cells co culture This study was designed to evaluate the efficacy of different somatic cell types and media in supporting in vitro maturation (IVM), in vitro fertilization (IVF) and early embryonic development competence of buffalo follicular oocytes. Cumulus oocyte complexes were collected for maturation from follicles (>6mm) of buffalo ovaries collected at the local abattoir. Oocytes were co-cultured in tissue culture medium (TCM-199) with either granulosa cells, cumulus cells, or buffalo oviductal epithelial cells (BOEC) @ 3x106 cells/ml or in TCM-199 without helper cells (control) at 39°C and 5%CO2 in humidified air. Fresh semen was prepared in modified Ca++ free Tyr...
Aim of this study was the comparison of in vitro embryo production in the buffalo species using two ...
Ham's F-10 with or without gonadotrophins and supplemented with either 20 % buffalo oestrous se...
This is the review of original data concerning the effect of some factors on oocyte development in v...
This work was carried out to evaluate the quality and the developmental competence of the in vitro b...
Availability of developmentally competent buffalo oocytes is critical for in vitro embryo production...
Abstract: Effect of co-culture of buffalo preantral follicles with different somatic cells, i.e. cum...
Effects of two maturation media (TCM-199 and Ham's F-12) with and without the addition of oestrus bu...
This work was carried out to develop a defined in vitro maturation medium for buffalo oocytes that w...
The present review intends to describe the state of the art of IVEP in buffalo species, emphasizing ...
Removal of cumulus cells is necessary for several technologies such as vitrification, intracytoplasm...
The identification of an optimal in vitro fertilization system is critical in order to improve the i...
Although removal of cumulus cells improves the efficiency of vitrification of buffalo (Bubalus bubal...
The present study aims to study the effects of epidermal growth factors (EGF) supplementations to th...
This study was undertaken in order to evaluate the effect of oocyte source (live animals and abattoi...
In vitro embryo production involves a combination of the techniques of in vitro maturation (IVM), fe...
Aim of this study was the comparison of in vitro embryo production in the buffalo species using two ...
Ham's F-10 with or without gonadotrophins and supplemented with either 20 % buffalo oestrous se...
This is the review of original data concerning the effect of some factors on oocyte development in v...
This work was carried out to evaluate the quality and the developmental competence of the in vitro b...
Availability of developmentally competent buffalo oocytes is critical for in vitro embryo production...
Abstract: Effect of co-culture of buffalo preantral follicles with different somatic cells, i.e. cum...
Effects of two maturation media (TCM-199 and Ham's F-12) with and without the addition of oestrus bu...
This work was carried out to develop a defined in vitro maturation medium for buffalo oocytes that w...
The present review intends to describe the state of the art of IVEP in buffalo species, emphasizing ...
Removal of cumulus cells is necessary for several technologies such as vitrification, intracytoplasm...
The identification of an optimal in vitro fertilization system is critical in order to improve the i...
Although removal of cumulus cells improves the efficiency of vitrification of buffalo (Bubalus bubal...
The present study aims to study the effects of epidermal growth factors (EGF) supplementations to th...
This study was undertaken in order to evaluate the effect of oocyte source (live animals and abattoi...
In vitro embryo production involves a combination of the techniques of in vitro maturation (IVM), fe...
Aim of this study was the comparison of in vitro embryo production in the buffalo species using two ...
Ham's F-10 with or without gonadotrophins and supplemented with either 20 % buffalo oestrous se...
This is the review of original data concerning the effect of some factors on oocyte development in v...