Reagents Materials

In semi-dry blotting the electrodes are placed directly in contact with the gel/nitrocellulose membrane sandwich to provide a fast, efficient transfer. The polyacrylamide gels must be equilibrated in transfer buffer, to remove electrophoresis buffer salts and detergents, and the nitrocellulose membranes and filter papers arepre-wetted, but that is all the buffer that is required (hence the term “semi-dry”). Using a platinum-coated titanium plate as the anode and a stainless-steel plate as the cathode, the Trans-Blot SD cell transfers in a horizontal configuration without a buffer tank or gel cassettes. Because of this direct contact there is a minimum of transfer buffer required (less than 200ml). It is important to exclude excess moisture and air bubbles trapped in the filter papers and membrane when setting up the transfer, usually a pipet rolled over the surface will take care of this, but other than that, the set-up for this process is extremely simple. Up to four mini gles can be transferred at the same time by placing them sidy-by-side on the anode platform