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Rosemary (Rosmarinus officinalis L., family Lamiaceae) had been reported in traditional medicine, to exhibit antimicrobial properties. Therefore, this study is aimed at determining the antibacterial activity of R. officinalis leaves against pathogenic microorganisms by determination the minimal inhibitory concentration and to serve as criteria to recommend the ethno pharmacological uses of the plant. Plant leaves were dried, powdered and extracted by cold maceration with methanol for 48h. The extracts were screened against 24h broth culture of bacteria seede