Catalog Number WGA3 Storage Temperature –20 °C TECHNICAL BULLETIN Product Description

method that allows the researcher to generate a representative, ~500-fold amplification of genomic DNA. The kit utilizes a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlexÒ Library molecules flanked by universal priming sites. The OmniPlex library is then PCR amplified using universal oligonucleotide primers and a limited number of cycles. The GenomePlex WGA Reamplification Kit allows subsequent reamplifications of the WGA product with little genetic bias. This kit contains an optimized enzyme that decreases the background in the reaction. The WGA DNA Polymerase successfully produces a negative control sample without product. This result provides more accuracy in downstream PCR or hybridization techniques. The format of this kit, with the dNTPs separated, allows the user to create custom amplification mixes by supplementing with alternative nucleotide blends. Reagents Provide